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TABLE 3-4  COMPLETED PROJECTS: IN SITU BIOLOGICAL TREATMENT FOR SOIL, SEDIMENT, BEDROCK AND SLUDGE
sitename and contact technology and vendor media treated contaminants treated operating parameters materials handling residuals management comments
Chemical Disposal Pit, Hill AFB, UT 

1998

James T. Gibbs 614-424-6424  
Bioventing

Battelle Columbus, OH
Soil TCE, DCB, BTEX  In situ   In situ “Bioventing Nonpetroleum Hydrocarbons,” 
Engineered Approaches for In Situ 
Bioremediation of Chlorinated Solvent 
Contamination. 1999. (p 7-14) 
Reilly Tar Superfund Site, St. Louis Park, MN 

1997

Dr. Paul McCauley 513-569-7444 mccauley.paul@epa.gov 
Bioventing

EPA National Risk Management Research Laboratory
Soil PAHs  In situ   In situ “Bioventing for Enhanced Degradation of PAHs,” 
Tech Trends, Aug 1999 
St. Louis Park, MN 

1997 

Paul McCauley 513-569-7444 
Bioventing

National Risk Management Research Laboratory Cincinnati, OH
Soil Hydrocarbons In situ   In situ Bioventing (Air-Injection) [An EPA SITE 
Program document will be produced.] 
Savannah River

DOE Program Manager
Kurt Gerdes
(301) 903-7289

Bioventing Soil & Ground water TCE and PCE. Soil:10 ppm, GW: 1 ppm
Disposal of solvents used to degrease nuclear fuel target elements.
  Levels attained < 2 ppb   Capital: $150K + 200 man-hours per week
Air Force Tech Demo - Program was launched in 5/92

Lt. Col. Ross N. Miller
(210) 536-4331

Bioventing initiative Soil Diesel, jet fuel, fuel oil, or petroleum hydrocarbons Aerobic degradation by direct injection or extraction of air Temporary shutdown of air injection in vent well to measure in situ rate of oxygen respiration in the monitoring wells. In situ technique for non- and semi-volatile hydrocarbons Degrada- tion up to 5,000 ppm/year. 
Apparatus is relatively non- intrusive.
Tyndall AFB, FL
Armstrong Laboratory /EQW
(904) 283-6208
DSN: 523-6208
Bioventing Soil Volatile hydrocarbons in vadose zone > 1,000 mg TPH/kg soil declined to < 30 mg TPH/kg soil In situ   In situ $15 -$20/m3 ($12 -$15/yd3
Eielson AFB, AK
Armstrong Laboratory /EQW
Kathy Vogel
Tyndall AFB, FL
(904) 283-6208 DSN: 523-6208
Bioventing Soil Volatile hydrocarbons In situ   In situ Average bioventing cost $10 -$15/yd3
Hill AFB, UT

AFCEE/Armstrong Laboratory /EQW Kathy Vogel (904)283-6208

Bioventing Soil to a depth of 60 ft. 25,000 gallons of JP-4. Starting levels at 20,000 ppm TPH. 98% reduction.

A pilot-scale demo is presently (1997) being performed to treat chlorinated solvents.

In situ   In situ Average bioventing cost $10-$15/yd3
Lowry AFB,

Lt.COL Tom Williams
3415 CES/DEV
Lowry AFB, CO 80230

Bioventing Soil Heating oil which contained relatively high concentration of TPH. In situ   In situ Capital: $28,500; Annual Operating: $32,875
DOI Tech Demo
(USGS) Galloway Township, NJ 1988

Herbert T. Buxton
(609) 771-3900

Vapor extraction and bioventing design Soil & ground water Gasoline AIRFLOW - an adaptation of the USGS ground water flow simulator MODFLOW to perform airflow simulations to predict well locations and pumping rates None Success dependent on ability to characterize 
air permeability.
Former Chemical Drum Storage Pad, Rickenbacker Air National Guard Base, OH 

1998 

Wendy J. Davis-Hoover 513-569-7206 davis-hoover.wendy@epa.gov 
In situ soil bio- remediation w/ Lasagna™ 



U.S. EPA Cincinnati
Soil TCE  In situ   In situ “In Situ Bioremediation Using 
Horizontal Lasagna™,” Engineered 
Approaches for In Situ Bioremediation 
of Chlorinated Solvent Contamination. 
1999. (p 263-267) 
Savannah River Site Sanitary Landfill, Aiken, SC 

1998

Robin L. Brigmon 
In situ soil bio- remediation/

Westinghouse Savannah River Co. Aiken, SC
Soil TCE, VC, DCE  In situ   In situ Evaluation of Methanotrophic Bacteria During 
Injection of Gaseous Nutrients for In Situ 
Trichloroethylene Bioremed-iation in a 
Sanitary Landfill WSRC-MS-98-00854 
Kennedy Space Flight Center, Cape Canaveral, FL 

1998 

Tom Rogers 409-693-0017 
In situ soil bio- remediation/

Lynntech, Inc. College Station, TX
Soil Chlorinated Solvents  In situ   In situ Resource Guide for Electrokinetics 
Laboratory & Field Processes Applicable to 
Radioactive & Hazardous Mixed Wastes in Soil & Groundwater ... EPA 402-R-97-006 (p 59)
Radford Army Ammunition Plant, VA 

1997 

Dr. Tom Rogers 409-693-0017 
In situ soil bio- remediation/

1997

Lynntech, Inc. College Station, TX
Soil Heavy Metals  In situ   In situ Resource Guide for Electrokinetics
Laboratory & Field Processes Applicable 
to Radioactive & Hazardous Mixed Wastes 
in Soil & Groundwater EPA 402-R-97-006 (p 56) 
EPA Remedial Action Seymour Recycling, IN

Summer 1990
8/86 to 10/86
1/87 to 2/87

Jeff Gore
(312) 886-6552

In situ soil bio- remediation/ ABB Environ- mental Services Soil (12 acres to 10 ft deep, about 43,500 yd3) 54 contaminants present, including TCE, TCA, and carbon tetrachloride. No standards or criteria for this OU in ROD Additives - nitrogen, phosphorus, potassium, sulfur as fertilizer (200,000 gallons of nutrients added) Tilling Capping in place The soil became saturated quickly during this project, 
creating surface pools. The specially designed 
tractor got stuck.
EPA Removal Action Roseville Drums, CA

2/12/88 to 11/9/88

Brad Shipley
(415) 744-2287

In situ bio- remediation/ EPA removal contractor Soil (14 yd3) Input:

Dichloro- benzene - 4,000 ppm

Phenol - 12,000 ppm

Additives to soil: manure, water Tilling Output:

Dichloro- benzene -140 ppm

Phenol - 6 ppm

Midnight dump on dirt road.
EPA Removal Action Gila River Indian Reservation, AZ

6/24/85 to 10/23/85

Richard Martin
(414) 744-2288

In situ anaerobic biological treatment (preceded by chemical treatment) /EPA removal contractor Soil (3,220 yd3) Toxaphene

Input: 470 ppm

Output: 180 ppm

pH: 8.3 to 9.8 Additives to soil: sulfuric acid, manure, sludge Tilling Capped in place The biological treatment would have been 
more successful if the neutralization after 
the chemical treatment had been more complete. 
Tearing of the plastic sheets covering the soils 
allowed air in and prevented anaerobic activity.
EPA Removal Action Gila River, Indian Reservation, AZ

3/28/85 to 6/24/85

Richard Martin (414) 744-2288

In situ chemical treatment (followed by anaerobic bio- remediation)/ EPA removal contractor Soil (3,200 yd3) Input:

Toxaphene - 1,470 ppm
Ethyl parathion - 86 ppm
Methyl parathion - 24 ppm

pH: 10.12 to 11.8 Moisture: wet additives to soil: sodium hydroxide, water Bio- remediation Output:

Toxaphene - 470 ppm
Ethyl parathion - 56 ppm
Methyl parathion - 3 ppm

Drum storage/disposal.
Navy Demo
Naval Communica- tion Station, Scotland

2/85 to 10/85

Deh Bin Chan
(805) 982-4191

Biodecontami- nation of fuel oil spills Soil Fuel Oil In situ; micro- organisms function best at 20-35 °C In situ In situ Diesel fuel storage tanks and piping.
DOE
Savannah River Site, SC

Terry C. Hazen
(803) 725-5178

Biodegradation Soil & ground water TCE, PCE declined to < 2 ppb In situ Injection of 1- 4% methane/air into aquifer via horizontal wells In situ Inhibited by copper or high clay content.
Army Demo
U.S. Army Construction Engineering Research Laboratory, IL

Jean Donnelly
(217) 352-6511

Biodegradation of oil contaminated soils Soil Motor oil/lubrication oil In situ Disk inoculant & nutrients into contaminated soil. Cover soil w/ventilated plastic sheeting. In situ Applicable to spills on air strips, roads, 
and parking lots.
Air Force Demo
Kelly AFB, TX & Eglin AFB, FL

Joe Laird
(402) 221-7772

In situ Biodegradation Soil & ground water Hydrocarbons -fuels, fuel oils, & non- halogenated solvents In situ soil conditioning and electron acceptor addition. Nutrients introduced into aquifer through irrigation wells Pumping wells remove excess fluids Site character- ization necessary to 
determine soil/chemical compatibility.
Formerly JimBo's Gas N'Goodies, Aiken, SC 

1999

Jim Ullery 706-722-3490 JUllery@setechctr.org 
In situ Biodegradation

Southeastern Technology Center (STC)
Soil BTEX  In situ   In situ PHOSter Bioremediation Technology: 
Award- Winning Technology Demonstrated in
Downtown Augusta
setechctr.org/Environmental/phoster.htm 
FAA Technical Center-Area D Atlantic County, NJ

Carla Struble
(212) 264-4595

Enhanced biodegradation Nutrient addition and ground water reinjection in saturated soil (sand) 33,000 yd3 Jet fuel NAPLs In situ   In situ Expected full scale $286K CAP and $200K O&M
Pinellas STAR Center, Largo, FL 

1997

M.F. DeFlaun 
Enhanced aerobic biodegradation

Envirogen, Inc. Lawrenceville, NJ
Soil Chlorinated Solvents  In situ   In situ Performance Evaluation of an In Situ Anaerobic Biotreatment System for Chlorinated Solvents EPA 600-A-98-041 
Eglin AFB, FL

Alison Thomas (904) 283-6303

Enhanced anaerobic biodegradation Fuel con- taminated aquifer. 4,000 ppb BTEX       Using nitrate as an alternative electron acceptor
French Limited Superfund Site, Crosby, TX

Judith Black
Remedial Project Manager
U.S. EPA Region 6
(214)665-6937

Slurry-phase bioremediation 300,000 tons of soil and sludge PCBs (up to 616 mg/kg), volatile organics (up to 400 mg/kg), penta- chlorophenol (up to 750 mg/kg), semivolatiles (up to 5,000 mg/kg) and metals (up to 5,000 mg/kg). In situ   In situ Total cost of $49,000,000 of which $26,900,000 
directly related to treatment, $16,500,000 for before- treatment, $5,600,000 for after- treatment.
DOE Demo
Savannah River Site, SC

Nate Ellis
(803) 952-4846
Brian Loony
(803) 752-5181

Vegetation enhanced biodegradation Soil TCE, PCE & PAHs at 10,000 ppb In situ Root- associated micro- organisms degrade contaminants. In situ Pine trees are most effective. Depth limited to 
about 20 ft. $50,000/ acre.
Brookhaven National Laboratory 

1998

USDOE 
Phyto- remediation Soil Cs(137)  In situ   In situ “Biomass Remediation System (TMS #251),”
SCFA Midyear Review Report and Supporting Documentation, 1999 
Whitewood Creek, SD 

1998

Jerald R. Schnoor 319-335-5649 jschnoor@cgrer.uiowa.edu 
Phyto- remediation


University of Iowa
Soil As, Cd  In situ   In situ Phytostabilization Demonstra-tion, 
One Acre Test Plot, Mine Wastes, 
Phytoremediation: Technology Evaluation Report. GWRTAC TE-98-01 (p 8) 
Dearing, KS 

1998 

G. Pierzynski 785-532-7209 gmp@ksu.edu  
Phyto- remediation

Kansas State University
Soil Pb, Zn, Cd  In situ   In situ Phytostabilization Demon-stration, 
One Acre Test Plot Abandoned Smelter, 
Barren Land, Phytoremediation: Technology Evaluation Report. GWRTAC TE-98-01 (p 8) 
Trenton, NJ Site 

1998 

Burt Ensley 908-438-0900 
Phyto- remediation

Phytotech, Inc. Monmouth, NJ
Soil Heavy Metals  In situ   In situ Phytoextraction of Metal from Soil 
[An EPA SITE Program document will be produced.] 
DOE Site, Ashtabula, OH 

1997 

Burt Ensley 908-438-0900 
Phyto- remediation

Phytotech, Inc. Monmouth, NJ
Soil Inorganic Compounds In situ   In situ Rhizofiltration Demonstration, DOE Energy Wastes, Phytoremediation: Technology Evaluation Report. GWRTAC TE-98-01 (p 8) 
Naval Surface Warfare Center, Dahlgren, Site 17 Phyto- remediation Soil and Groundwater Mercury (<0.14 ug/L) Hybrid Poplar Trees
Evergreen Trees
  Pre-design  
Idaho National Engineering Laboratory (USDOE, Operable Unit 21) Phyto- remediation Soil Chromium (NR) Cesium-137 (NR) Mercury (NR) Selenium (NR) Silver (NR) Zinc (NR) Prairie Cascade Willows
Kochia Scoparia
  Operational  
Bofors Nobel (Operable Unit 1) Phyto- remediation Soil, Sludge, and Groundwater Benzene No Information   Pre-design  
Boarhead Farm Phyto- remediation Soil and Groundwater Cadmium (5 ug/L,
Groundwater)
Nickle (100 ug/L,
Groundwater)
Benzene (0.5 mg/kg, Soil)
Trichloroethene (0.4 mg/kg, Soil)
No Information   Design  
Aberdeen Proving Grounds (Edewood Area, J-Field Soil OU Phyto- remediation Soil and Groundwater 1,1,2,2-Tetrachloroethene (NR)
Trichloroethane (NR)
Hybrid Poplar Trees
Magnolia Trees
Silver Maple Trees
  Operational  
McCormick & Baxter SUPERFUND Site, Portland, OR (Wood treatment site)

Steve Rock (513) 569-7149

Phyto- remediation Shallow soil PCP and PAHs Perennial ryegrass Site seeded and irrigation system installed    
Argonne National Laboratory

M. Christina Negri, DOE, Office of Energy Research, (630) 252-9662

Phyto- remediation Soils Heavy metals       Project is also investigating the fate of 
chlorinated organic solvents in plant systems
Craney Island Fuel Terminal, U.S. Navy, Portsmouth, VA

Dr. Stephanie Fiorenza, DoD/AATDF (713) 527-8101 x3338

Phyto- remediation Soils (15 acres) TPH 1) bermuda grass sod over-seeded with rye grass, 2) white clover, 3) tall fescue The site is visited once per month during the growing season. Plots are weeded, mowed, and fertilized. Sampling is conducted monthly or bimonthly for total petroleum hydrocarbons (TPH) and nutrients in soil, above- and below- ground biomass development, and contaminants in soil pore water.    
EPA S.I.T.E. Program, Ogden, Utah

Steve Rock (513) 569-7149

Phyto- remediation; Chevron Research, Phytokinetics, Inc Soil and ground water Petroleum spill Poplar trees, juniper trees, alphalfa, and fescue Planted in three rows (six feet apart) perpendicular to ground water flow to form subsurface interceptor barrier to the saturated zone   Plants used: Alfalfa, Poplar Juniper, Fescue
Ohio (Former metal plating site)

Steve Rock (513) 569-7149

Phyto- remediation; Phytotech, Inc Soil Lead, chromium, and hexavalent chrome Indian mustard plants Two to three plantings per year are expected Plants will be harvested, then either disposed of or recycled for metals content  

Sources: Innovative Treatment Technologies: Annual Status Report (EPA, Eighth Edition, Nov. 1996). Completed North American Innovative Remediation Technology Demonstration Projects (EPA, Aug.1996)